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A Signal‐Amplified Electrochemical Immunosensor Based on Prussian Blue and Pt Hollow Nanospheres as Matrix
Author(s) -
Liu Hong,
Yu Rongjie,
Peng Kanfu,
Zhao Hongwen,
Li Lian,
Wu Xiongfei
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000172
Subject(s) - prussian blue , detection limit , reproducibility , immunoassay , electrochemistry , electrode , materials science , matrix (chemical analysis) , chemistry , nuclear chemistry , chromatography , nanotechnology , antibody , immunology , biology
Through electrodepositing Prussian blue (PB) and chitosan (CS), then casting Pt hollow nanospheres (HN‐Pt) and assembling CA19‐9 antibody on the electrode surface, an immunosensor was achieved. A new signal amplification strategy based on PB and HN‐Pt toward the electrocatalytic reduction of H 2 O 2 was employed when performing the determination. The resulting immunosensor showed a high sensitivity, broad linear response to carbohydrate antigen 19‐9 (CA19‐9) in two ranges from 0.5 to 30 and 30 to 240 U mL −1 with a low detection limit of 0.13 U mL −1 ( S / N =3). Moreover, it displayed good reproducibility and stability, and would be potentially attractive for clinical immunoassay of CA19‐9.