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Electrochemical Immunosensing Chip Using Selective Surface Modification, Capillary‐Driven Microfluidic Control, and Signal Amplification by Redox Cycling
Author(s) -
Kim ByungKwon,
Yang SangYoun,
Aziz Md. Abdul,
Jo Kyungmin,
Sung Daekyung,
Jon Sangyong,
Woo Han Young,
Yang Haesik
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000148
Subject(s) - microfluidics , capillary action , electrochemistry , fluidics , electrode , detection limit , chemistry , nanotechnology , chip , biosensor , redox , materials science , analytical chemistry (journal) , chromatography , inorganic chemistry , electrical engineering , composite material , aerospace engineering , engineering
A sensitive electrochemical immunosensing chip is presented by employing (i) selective modification of protein‐resistant surfaces; (ii) fabrication of a stable Ag/AgCl reference electrode; (iii) capillary‐driven microfluidic control; (iv) signal amplification by redox cycling along with enzymatic reaction. Purely capillary‐driven microfluidic control is combined with electrochemical sandwich‐type immunosensing procedure. Selective modification of the surfaces is achieved by chemical reactivity‐controlled patterning and electrochemical deposition. Fluidic control of the immunosensing chip is achieved by spontaneous capillary‐driven flows and passive washing. The detection limit for mouse IgG in the immunosensing chip is 10 pg/mL.