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New Electroactive Hybridization Indicators 2‐Phthalimido‐ N ‐Substitutedphenylethanesulfonamide Derivatives for Biosensor Applications: Ring Substituent Effect on Interaction between Compound and DNA
Author(s) -
OzkanAriksoysal Dilsat,
Akgul Ozlem,
Aydinlik Seyma,
Topkaya Seda Nur,
Aladag Nilay,
Ozsoz Mehmet
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000128
Subject(s) - substituent , chemistry , differential pulse voltammetry , guanine , dna , electrochemistry , ring (chemistry) , combinatorial chemistry , biosensor , carbon paste electrode , cyclic voltammetry , medicinal chemistry , photochemistry , stereochemistry , electrode , organic chemistry , biochemistry , nucleotide , gene
In this work, an electrochemical DNA‐based sensor was developed for the detection of the interaction between the anticonvulsant compounds 2‐phthalimido‐ N ‐substituted phenylethanesulfonamides (PMPES‐derivatives) and 24‐mer short DNA sequences by using differential pulse voltammetry (DPV) based on both compound and guanine oxidation signals at the renewable carbon graphite electrode (CGE) surface. The influence of compounds on DNA showed differences depending on the nature and position of the substituent on the N ‐phenyl ring. Compounds bearing 3‐methoxy, 4‐chloro and 2,6‐dimethyl substituents bind to single stranded probe DNA more strongly than the other derivatives of PMPES. Thus, these compounds were evaluated for use as an electrochemical hybridization label (indicator).