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Electrochemical Measurements on Supported Phospholipid Bilayers: Preparation, Properties and Ion Transport Using Incorporated Ionophores
Author(s) -
Navrátil Tomáš,
Šestáková Ivana,
Štulík Karel,
Mareček Vladimír
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000061
Subject(s) - ionophore , chemistry , valinomycin , electrochemistry , membrane , phospholipid , dielectric spectroscopy , ion transporter , ion , lipid bilayer , inorganic chemistry , potentiometric titration , cadmium , nuclear chemistry , analytical chemistry (journal) , chromatography , electrode , organic chemistry , biochemistry
Abstract Model phospholipid bilayers (PLBs) were prepared from 1,2‐dipalmitoyl‐ sn ‐glycero‐3‐phosphocholine (DPPC) and 1,2‐dipalmitoyl‐ sn ‐glycero‐3‐phosphoethanolamine (DPPE) on a polycarbonate support and characterized by electrochemical impedance spectroscopy (EIS) measurements. The PLBs were formed by self‐assembling in the 8.0 μm holes of the Isopore Membrane Filters, ionophores valinomycin and calcimycin (A23187) were incorporated. The effect of the applied voltage was monitored and reversible changes were observed. The ion transport across supported PLBs (SPLB) from DPPC was studied voltammetrically on the example of the cadmium ion and the ionophore calcimycin. Complex behavior was observed for cadmium ion transport with this ionophore in the presence of calcium ions.