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Comparative Analysis of Proline Enantiomer in Chiral Recognition of Biological Macromolecule in Immunoassay
Author(s) -
Chen Min,
Fu Yingzi,
Cui Xin,
Wang Lilan,
Li Miaosi
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200904688
Subject(s) - cyclic voltammetry , enantiomer , macromolecule , amperometry , dielectric spectroscopy , chemistry , carcinoembryonic antigen , proline , amino acid , electrochemistry , electrode , combinatorial chemistry , materials science , organic chemistry , biochemistry , biology , cancer , genetics
An interesting phenomenon of the stereoselective interaction between biological macromolecule and amino acid enantiomorphous was described. Firstly, proline enantiomer ( L‐ and D‐ proline) was assembled on the glassy carbon electrode surface. Then carcinoembryonic antibody (anti‐CEA) was loaded to the enantiomer surface, electrochemical impedance spectroscopy (EIS) was used to monitor the growth of amino acid film. The assembly process was characterized by cyclic voltammetry (CV) and atomic force microscopy (AFM) was applied to image the chiral films. Finally, the developed electrodes had interacted with carcinoembryonic antigen (CEA) in varying concentration solutions. The AFM and amperometric results revealed that the proline enantioner had the chiral recognition function to antibody.