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The Use of an Electroactive Marker as a SERS Label in an E ‐melting Mutation Discrimination Assay
Author(s) -
Mahajan Sumeet,
Richardson James,
Gaied Nouha Ben,
Zhao Zhengyun,
Brown Tom,
Bartlett Philip N.
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200904681
Subject(s) - anthraquinone , oligonucleotide , point mutation , materials science , melting point , chemistry , voltammetry , dna , mutation , microbiology and biotechnology , combinatorial chemistry , analytical chemistry (journal) , chromatography , electrochemistry , biology , biochemistry , electrode , organic chemistry , gene
In this paper we describe the first example of the use of an electroactive label as a SERS marker for detecting and quantifying target oligonucleotides and distinguishing mutations using a combination of voltammetry and electrochemically induced melting ( E‐ melting). The experiments were carried out on sphere segment void substrates selected to show strong surface enhancement for SERS at 633 nm. DNA analysis was carried out for CFTR sequences using synthetic 22‐mer oligonucleotides labelled with a modified anthraquinone at the 3′‐end. Discrimination between the wild type, 1653C/T (point mutation) and ΔF508 (triple deletion) is demonstrated.