Premium
Laccase Based Synergistic Electrocatalytical System
Author(s) -
Kulys Juozas,
Vidziunaite Regina
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200904679
Subject(s) - phenoxazine , phenothiazine , chemistry , substrate (aquarium) , laccase , reaction rate constant , kinetics , combinatorial chemistry , inorganic chemistry , organic chemistry , enzyme , medicine , oceanography , physics , quantum mechanics , pharmacology , geology
Bioelectrodes employing the conversion of N ‐substituted phenothiazine/hexacyanoferrate(II) and N ‐substituted phenoxazine/hexacyanoferrate(II) that act as synergistic substrates were built using graphite electrode and recombinant laccase. The response of the bioelectrodes to low reactive substrate (hexacyanoferrate(II)) increases 2.9–97 times in presence of high reactive phenothiazine or phenoxazine mediator. The sensitivity of the bioelectrodes reaches 0.5–14.3 A/M⋅cm 2 . To background a scheme of bioelectrodes action the kinetics of laccase‐catalyzed synergistic substrates oxidation was investigated in homogenous solution. A satisfactory agreement of experimentally determined rate constants and calculated using parameters of bioelectrocatalytical system confirms the scheme of bioelectrodes action.