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Ultrasensitive Hydrogen Peroxide Biosensor Based on Enzyme Bound to Layered Nonoriented Multiwall Carbon Nanotubes/Polyelectrolyte Electrodes
Author(s) -
Munge Bernard S.,
Dowd Richard S.,
Krause Colleen E.,
Millord Lines N.
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200904676
Subject(s) - hydrogen peroxide , cyclic voltammetry , carbon nanotube , biosensor , horseradish peroxidase , electron transfer , detection limit , electrode , electrochemistry , materials science , calibration curve , analytical chemistry (journal) , chemistry , nuclear chemistry , inorganic chemistry , nanotechnology , photochemistry , chromatography , organic chemistry , enzyme
A sensitive hydrogen peroxide sensor based on horseradish peroxidase covalently attached to layered nonoriented MWNTs modified electrode is presented. Cyclic voltammetry results gave quasi‐reversible Fe III /Fe II voltammetry. The electron transfer rate constant ( k s ) and Michaelis–Menten constant ( K M ) in pH 7 is 48.8±0.9 s −1 and 0.13±0.05 mM respectively. A linear calibration curve for hydrogen peroxide was obtained up to 120 nM under the optimized conditions with a remarkable detection limit of ( S / N =3) 1.5 nM. Results suggest that the nonoriented nanotubes act as electrical conductors and may also provide large surface area facilitating facile electron transfer and excellent electrochemical catalysis.