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Voltammetric Determination of Azidothymidine Using Silver Solid Amalgam Electrodes
Author(s) -
Pecková Karolina,
Navrátil Tomáš,
Yosypchuk Bogdan,
Moreira Josino Costa,
Leandro Katia Christina,
Barek Jiří
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200904660
Subject(s) - hanging mercury drop electrode , mercury (programming language) , amalgam (chemistry) , electrode , chemistry , voltammetry , detection limit , differential pulse voltammetry , chromatography , analytical chemistry (journal) , materials science , electrochemistry , cyclic voltammetry , computer science , programming language
A new simple and direct electroanalytical method was developed for the determination of azidothymidine in commercial pharmaceutical preparations. It is based on differential pulse voltammetry at silver solid amalgam electrode with polished surface (p‐AgSAE) or surface modified by mercury meniscus (m‐AgSAE). The electroreduction of azidothymidine in basic media at these electrodes gives rise to one irreversible cathodic peak. Its potential in 0.05 mol L −1 borate buffer, pH 9.3 at ca. −1050 mV is comparable to that using hanging mercury drop electrode (HMDE). Achieved limits of quantitation are in the 10 −7 mol L −1 concentration range for both amalgam electrodes. According to the procedure based on the standard addition technique, the recoveries of known amounts of azidothymidine contained in pharmaceutical preparations available in capsules were 101.4±1.8% (m‐AgSAE), 100.3±3.5% (p‐AgSAE) and 102.0±1.0% (HMDE) ( n =10). There was no significant difference between the values gained by proposed voltammetric methods and the HPLC‐UV recommended by the United States Pharmacopoeia regarding the mean values and standard deviations.