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Electrochemical Sensing of NADH and Glutamate Based on Meldola Blue in 1,2‐Diaminobenzene and 3,4‐Ethylenedioxythiophene Polymer Films
Author(s) -
Doaga Rodica,
McCormac Timothy,
Dempsey Eithne
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200904627
Subject(s) - amperometry , electrochemistry , electrocatalyst , detection limit , chemistry , nuclear chemistry , glutamate dehydrogenase , conductive polymer , polymer , redox , glutamate receptor , chromatography , electrode , inorganic chemistry , organic chemistry , biochemistry , receptor
The redox mediator Meldola blue (MB) was entrapped into two polymers, poly‐1,2‐diaminobenzene (p‐DAB) and poly‐3,4‐ethylenedioxythiophene (p‐EDOT) by potential cycling and films were applied to NADH oxidation with subsequent glutamate detection using immobilized glutamate dehydrogenase. Both polymer films were tested for electrocatalysis of NADH using amperometry at E app =0.1 V vs. Ag/AgCl and similar response characteristics were obtained with sensitivity values of 6.1 nA μM −1 , linear range up to 0.5 mM ( R 2 =0.9972) and LOD of 50 μM. Subsequent amperometric determination of glutamate resulted in sensitivity 0.7 nA μM −1 , linearity 0–100 μM and detection limit of 2 μM glutamate.