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Quantitative Photoelectrochemical Detection of Biotin Conjugated CdSe/ZnS Quantum Dots on the Avidin Immobilized ITO Electrodes
Author(s) -
Baş Deniz,
Boyacı İsmail Hakkı
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200904619
Subject(s) - photocurrent , quantum dot , avidin , photoelectrochemistry , conjugated system , biosensor , photoelectrochemical cell , electrode , materials science , biotin , linear range , electron transfer , optoelectronics , photochemistry , chemistry , nanotechnology , detection limit , electrolyte , electrochemistry , chromatography , biochemistry , composite material , polymer
Abstract Photocurrent generation from CdSe/ZnS (core/shell) quantum dots (QDs) in a photoelectrochemical cell was proposed to perform a bioaffinity biosensor in this study. The photocurrent of QDs is reversible and methylene blue as an electron transfer mediator causes a four‐fold increase in the photocurrent. We further present quantitative photoelectrochemical detection of biotin conjugated QDs on the avidin immobilized ITO electrodes. A linear calibration graph was obtained in the range of 4 and 18 nM of biotin conjugated QDs with a coefficient of determination of 0.997. Results imply that QDs can be successfully used as photoelectroactive labels for the photoelectrochemical biosensor systems.