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Reagentless Aptamer Based Impedance Biosensor for Monitoring Adenosine
Author(s) -
Liu Zhimin,
Li Zhejian,
Shen Guoli,
Yu Ruqin
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200904599
Subject(s) - biosensor , dielectric spectroscopy , aptamer , electron transfer , adenosine , chemistry , analytical chemistry (journal) , electrical impedance , redox , selectivity , linear range , materials science , electrochemistry , nanotechnology , electrode , detection limit , chromatography , inorganic chemistry , photochemistry , organic chemistry , biochemistry , engineering , biology , electrical engineering , genetics , catalysis
A simple and highly sensitive electrochemical impedance spectroscopy (EIS) biosensor based on nano‐MnO 2 as a platform for the immobilization of the aptamer was developed for the determination of adenosine. In the measurement of adenosine, the change in interfacial electron transfer resistance ( R et ) of the biosensor using a redox couple of [Fe(CN) 6 ] 3−/4− as the probe was monitored. The change of the electron transfer resistance (Δ R et ) of the biosensor was linear with the concentration of adenosine in the range from 1.0 nM to 100 nM. The fabricated sensor was shown to exhibit high sensitivity, desirable selectivity and good stability.