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Self‐Assembled Multilayers of Polyethylenimine and DNA: Spectrophotometric and Electrochemical Characterization and Application for the Determination of Acridine Orange Interaction
Author(s) -
Ferreyra N. F.,
Rivas G. A.
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200904593
Subject(s) - polyethylenimine , acridine orange , supramolecular chemistry , electrochemistry , chemistry , electrode , combinatorial chemistry , dna , cyclic voltammetry , acridine , chromophore , voltammetry , photochemistry , nuclear chemistry , organic chemistry , molecule , biochemistry , apoptosis , transfection , gene
This work deals with the study of the interaction between acridine orange (AO) and calf‐thymus double stranded DNA (dsDNA) present in supramolecular architectures built on gold electrodes modified with mercapto‐1‐propanesulfonic acid (MPS) by self‐assembling of polyethylenimine and dsDNA. The optimal conditions for building the supramolecular architecture were obtained from UV‐vis spectrophotometric experiments. The electrochemical studies were performed by adsorptive transfer square wave voltammetry from the evaluation of the oxidation signal of AO accumulated within the multistructure. The effect of the number of PEI‐dsDNA bilayers (Au/MPS/(PEI‐dsDNA) n ) on the accumulation and electrooxidation of AO is also discussed.