Premium
Glucosinolate Amperometric Bienzyme Biosensor Based on Carbon Nanotubes‐Gold Nanoparticles Composite Electrodes
Author(s) -
Serafín V.,
Agüí L.,
YáñezSedeño P.,
Pingarrón José M.
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200904574
Subject(s) - biosensor , myrosinase , amperometry , glucose oxidase , glucosinolate , sinigrin , carbon nanotube , chemistry , nanoparticle , colloidal gold , chromatography , electrode , nanotechnology , materials science , electrochemistry , agronomy , biology , brassica
A novel electrochemical biosensor design for glucosinolate determination involving bulk‐incorporation of the enzymes glucose oxidase and myrosinase into a colloidal gold ‐ multiwalled carbon nanotubes composite electrode using Teflon as binder is reported. Myrosinase catalyzes the hydrolysis of glucosinolate forming glucose, which is enzymatically oxidized. The generated hydrogen peroxide was electrochemically detected without mediator at the nanostructured composite electrode at E =+0.5 V vs. Ag/AgCl. Under the optimized conditions, the bienzyme MYR/GOx‐Au coll ‐MWCNT‐Teflon exhibited improved analytical characteristics for the glucosinolate sinigrin with respect to a biosensor constructed without gold nanoparticles, i.e. a MYR/GOx‐MWCNT‐Teflon electrode, as well as with respect to other glucosinolate biosensor designs reported in the literature. The biosensor exhibits good repeatability of the amperometric measurements and good interassay reproducibility. Furthermore, the biosensor exhibited a high selectivity with respect to various potential interferents. The usefulness of the biosensor was evaluated by the determination of glucosinolate in Brussel sprout seeds.