Premium
Construction of a Novel Glucose Biosensor Based on Covalent Immobilization of Glucose Oxidase on Poly(glycidyl methacrylate‐ co ‐vinylferrocene)
Author(s) -
Şenel Mehmet,
Abasıyanık M. Fatih
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900644
Subject(s) - glycidyl methacrylate , glucose oxidase , biosensor , copolymer , methacrylate , chemistry , polymer chemistry , amperometry , electron transfer , covalent bond , immobilized enzyme , combinatorial chemistry , electrochemistry , electrode , materials science , polymer , organic chemistry , enzyme , biochemistry
A novel glucose biosensor was constructed via direct covalent attachment of glucose oxidase onto epoxy group containing polymeric electron transfer mediator, Poly(glycidyl methacrylate‐ co ‐vinylferrocene). A copolymer of glycidyl methacrylate (GMA) and vinylferrocene (VFc) with different molar ratios has been prepared by free radical copolymerization. These copolymers have been utilized as polymeric mediators for amperometric glucose sensing. The catalytic electrochemistry of the enzyme electrode with the copolymer was investigated. Copolymer acts as an electron transfer mediator between the redox center of Glucose oxidase (GOx) and the electrode. The stability, reusability, pH and temperature response of the biosensor as well as its kinetic parameter have also been studied.