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Voltammetric, UV–Vis Spectrometric and Fluorescence Study of the Interaction of Ractopamine and DNA with the Aid of Multivariate Curve Resolution‐Alternating Least Squares
Author(s) -
Ni Yongnian,
Wang Yingxia,
Kokot Serge
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900596
Subject(s) - ractopamine , chemistry , analytical chemistry (journal) , differential pulse voltammetry , resolution (logic) , fluorescence spectroscopy , dna , fluorescence , cyclic voltammetry , chromatography , electrode , biochemistry , electrochemistry , physics , quantum mechanics , artificial intelligence , computer science
Interaction of a steroid drug, Ractopamine (RAC), and DNA was investigated by electroanalysis‐differential pulse and cyclic voltammetry (DPV and CV), and UV‐vis and fluorescence spectroscopy. DPV showed that RAC intercalated with DNA, and CV indicated that the reaction mechanism of RAC and dsDNA involved irreversible oxidation with the loss of two H + and a transfer of two electrons. Reaction binding parameters were obtained. Pure spectra of RAC, DNA and the RAC‐DNA complex, and their concentrations were extracted by multivariate curve resolution‐alternating least squares method (MCR‐ALS). Concentration profiles indicated quantitatively the course of the reaction.