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Label‐Free Electrochemical Thrombin Aptasensor Based on Ag Nanoparticles Modified Electrode
Author(s) -
Suprun Elena,
Shumyantseva Victoria,
Rakhmetova Svetlana,
Voronina Svetlana,
Radko Sergey,
Bodoev Nikolay,
Archakov Alexander
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900594
Subject(s) - aptamer , detection limit , thrombin , electrode , electrochemistry , silver nanoparticle , linear range , nanoparticle , materials science , chemistry , redox , nanotechnology , combinatorial chemistry , analytical chemistry (journal) , chromatography , inorganic chemistry , platelet , immunology , biology , genetics
A novel label‐free electrochemical method for protein detection based on redox properties of silver was developed. As recognition elements, thrombin‐binding aptamers were used. Screen printed electrodes modified with silver nanoparticles (AgNP) were employed as a sensing platform for aptasensor devices. The oxidation of silver upon polarization served as a basis for analytical response. Three different thrombin binding aptamers with various surface concentrations were studied. Linear range of aptasensor response corresponded to the 10 −9 M to 10 −7 M thrombin concentration range and the detection limit was 10 −9 M.