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Sensitive Superoxide Biosensor Based on Silicon Carbide Nanoparticles
Author(s) -
RafieePour HossainAli,
Noorbakhsh Abdollah,
Salimi Abdollah,
Ghourchian Hedayatollah
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900577
Subject(s) - biosensor , amperometry , detection limit , electron transfer , silicon carbide , nanoparticle , superoxide , chemistry , analytical chemistry (journal) , nuclear chemistry , redox , superoxide dismutase , materials science , electrochemistry , nanotechnology , inorganic chemistry , photochemistry , electrode , chromatography , enzyme , organic chemistry
Direct electron transfer of immobilized superoxide dismutase (Cu, Zn‐SOD) onto silicon carbide (SiC) nanoparticles displays a pair of well defined and nearly reversible redox peaks with formal potential ( E° ′) of −0.03 V in pH 7.4. The heterogeneous electron transfer rate constant ( k s ) and surface coverage (Γ) of immobilized SOD are 11.0±0.4 s −1 and 1.42×10 −11 mol cm −2 . Biosensor shows fast amperometric response (3s) with sensitivity and detection limit of 1.416 nA μM −1 , 1.66 μM, and 1.375 nA μM −1 , 2.1 μM for cathodically or anodically detection of superoxide, respectively. This biosensor also exhibits good stability, reproducibility and long life‐time.