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Determination of the Apparent Michaelis Constant of Glucose Oxidase Immobilized on a Microelectrode with Respect to Oxygen
Author(s) -
Yasukawa Tomoyuki,
Goto Keisuke,
Mizutani Fumio
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900501
Subject(s) - microelectrode , glucose oxidase , michaelis–menten kinetics , chemistry , hydrogen peroxide , oxygen , clark electrode , electrode , electrochemistry , electrolysis , immobilized enzyme , limiting oxygen concentration , analytical chemistry (journal) , inorganic chemistry , chromatography , enzyme assay , biochemistry , enzyme , electrolyte , organic chemistry
We report determination of the apparent Michaelis constant of glucose oxidase (GOx) immobilized on a microelectrode with respect to oxygen. We used a GOx‐modified microelectrode as a probe for scanning electrochemical microscopy. We detected hydrogen peroxide generated by the enzyme reaction at the microelectrode under controlling the oxygen concentration using water electrolysis at an interdigitated array (IDA) electrode. The response depends on the oxygen concentration, which is regulated by the microelectrode position and the potential applied to the IDA electrode. We estimated the apparent Michaelis constant with respect to oxygen in this experimental condition to be about 0.28 mM.