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Improved Performance of an Amperometric Biosensor with Polydiaminonaphthalene on Electrochemically Deposited Au Nanoparticles
Author(s) -
Noh HuiBog,
Kumar Pankaj,
Biswas Tapan Kumar,
Kim DukSoo,
Shim YoonBo
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900484
Subject(s) - dielectric spectroscopy , detection limit , cyclic voltammetry , x ray photoelectron spectroscopy , colloidal gold , amperometry , analytical chemistry (journal) , materials science , biosensor , covalent bond , nanoparticle , electrochemistry , quartz crystal microbalance , buffer solution , nuclear chemistry , chemistry , electrode , chromatography , adsorption , chemical engineering , nanotechnology , organic chemistry , engineering
The performance of an enzyme sensor fabricated through covalent bond formation on the HRP‐bonded poly(1,8‐diaminonaphthalene) (polyDAN) layer with gold nanoparticles (AuNPs) was applied to catalyze the electrochemical reduction of H 2 O 2 . The surface characteristics of the sensor probe were studied using cyclic voltammetry, SEM, XPS, QCM, and impedance spectroscopy. The AuNP‐deposited surface resulted in higher conductivity and sensitivity for H 2 O 2 detection in phosphate buffer solution. A linear calibration plot was obtained in the H 2 O 2 concentration range between 10.0 μM and 25.0 mM with detection limit 5.0±1.25 μM. The lifetime of HRP/polyDAN/AuNP/GC probe was over 70 days without response loss.