Nanostructured Iron Oxide Platform for Impedimetric Cholesterol Detection

A nanostructured iron oxide (NanoFe 3 O 4 , particle size ca. 25 nm and roughness ca. 21 nm) film deposited onto a hydrolyzed indium‐tin‐oxide (ITO) coated glass plate has been used to immobilize cholesterol oxidase (ChOx) to fabricate an impedimetric cholesterol sensor. Electrochemical studies reveal that surface charged Fe 3 O 4 nanoparticles provide better conformation for ChOx loading resulting in enhanced electron transfer between ChOx and the electrode. Impedimetric response studies of the ChOx/NanoFe 3 O 4 /ITO bioelectrode exhibit improved linearity (2.5–400 mg/dL), low detection limit (0.25 mg/dL), fast response time (25 s), high sensitivity (86 Ω/mg dL −1 /cm −2 ) and a low value of the Michaelis‐Menten constant ( K m , 0.8 mg/dL) with a regression coefficient of 0.997.