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Nanostructured Iron Oxide Platform for Impedimetric Cholesterol Detection
Author(s) -
Kaushik Ajeet,
Solanki Pratima R.,
Kaneto Keiichi,
Kim C. G.,
Ahmad Sharif,
Malhotra Bansi D.
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900468
Subject(s) - cholesterol oxidase , indium tin oxide , detection limit , oxide , nuclear chemistry , materials science , nanoparticle , electrode , electrochemistry , analytical chemistry (journal) , chemistry , nanotechnology , chromatography , cholesterol , metallurgy , biochemistry
A nanostructured iron oxide (NanoFe 3 O 4 , particle size ca. 25 nm and roughness ca. 21 nm) film deposited onto a hydrolyzed indium‐tin‐oxide (ITO) coated glass plate has been used to immobilize cholesterol oxidase (ChOx) to fabricate an impedimetric cholesterol sensor. Electrochemical studies reveal that surface charged Fe 3 O 4 nanoparticles provide better conformation for ChOx loading resulting in enhanced electron transfer between ChOx and the electrode. Impedimetric response studies of the ChOx/NanoFe 3 O 4 /ITO bioelectrode exhibit improved linearity (2.5–400 mg/dL), low detection limit (0.25 mg/dL), fast response time (25 s), high sensitivity (86 Ω/mg dL −1 /cm −2 ) and a low value of the Michaelis‐Menten constant ( K m , 0.8 mg/dL) with a regression coefficient of 0.997.