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Direct Electrochemistry and Electrocatalysis of Hemoglobin Immobilized in a Polymeric Ionic Liquid Film
Author(s) -
Zhang Qian,
Lv Xue,
Qiao Yun,
Zhang Ling,
Liu DaLiang,
Zhang Wei,
Han GuangXi,
Song XiMing
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900430
Subject(s) - ionic liquid , electrocatalyst , electrochemistry , vinyl alcohol , biosensor , electron transfer , redox , polymer , detection limit , chemistry , electrode , bioelectrochemistry , linear range , polyvinyl alcohol , chemical engineering , polymer chemistry , materials science , inorganic chemistry , catalysis , nanotechnology , organic chemistry , chromatography , engineering
A polymer film based on polymeric ionic liquid, which was poly(1‐vinyl‐3‐butylimidazolium chloride) (poly(ViBuIm + Cl − )for short), was firstly used as matrix to immobilize hemoglobin (Hb). FTIR and UV‐vis spectra demonstrated that the native structure of Hb was well preserved after entrapped into the polymer film. The Hb immobilized in the poly(ViBuIm + Cl − ) film showed a fast direct electron transfer for the Hb‐Fe III /Fe II redox couple. Based on the direct electron transfer of the immobilized Hb, polyvinyl alcohol (PVA)/Hb/poly(ViBuIm + Cl − )/GC electrode displayed good sensitivity and wide linear range for the detection of H 2 O 2 . The linear range of the PVA/Hb/poly(ViBuIm + Cl − )/GC electrode to H 2 O 2 is from 3.5 to 224 μM with a limit of detection of 1.17 μM. Such an avenue, which integrated polymeric ionic liquid and redox protein via a simple method, may provide a novel and efficient platform for the fabrication of biosensors, biofuel cells and other bioelectrochemical devices.