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A MgO Nanoparticles Composite Matrix‐Based Electrochemical Biosensor for Hydrogen Peroxide with High Sensitivity
Author(s) -
Lu Limin,
Zhang Li,
Zhang Xiaobing,
Wu Zaisheng,
Huan Shuangyan,
Shen Guoli,
Yu Ruqin
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900429
Subject(s) - biosensor , nanocomposite , amperometry , hydroquinone , hydrogen peroxide , nanoparticle , horseradish peroxidase , matrix (chemical analysis) , chitosan , nuclear chemistry , electrochemistry , detection limit , chemistry , chemical engineering , materials science , electrode , analytical chemistry (journal) , nanotechnology , chromatography , organic chemistry , engineering , enzyme
A novel horseradish peroxidase (HRP) electrochemical biosensor based on a MgO nanoparticles (nano‐MgO)‐chitosan (chit) composite matrix was developed. The morphology of nano‐MgO‐chit nanocomposite was examined by scanning electron microscopy (SEM). The interaction between nano‐MgO‐chit nanocomposite matrix and enzyme was characterized with UV‐vis spectra. This proposed composite material combined the advantages of inorganic nanoparticles and organic polymer chit. The HRP immobilized in the nanocomposite matrix displayed excellent electrocatalytic activity to the reduction of H 2 O 2 in the presence of hydroquinone as a mediator. The effects of the experimental variables such as solution pH and the working potential were investigated using steady‐state amperometry. The present biosensor (HRP‐modified electrode) had a fast response towards H 2 O 2 (less than 10 s), and excellent linear relationships were obtained in the concentration range of 0.1–1300 μM, with a detection limit of 0.05 μM ( S / N =3). Moreover, the stability and reproducibility of this biosensor were evaluated with satisfactory results.