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Parallel Detection of Different DNA Sequences on One Gold Electrode
Author(s) -
Bütow Sandra,
Lisdat Fred
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900426
Subject(s) - methylene blue , ferrocene , redox , electrode , electrochemistry , dna , hybridization probe , steric effects , combinatorial chemistry , chemistry , methylene , molecule , nanotechnology , inorganic chemistry , materials science , stereochemistry , biochemistry , organic chemistry , photocatalysis , catalysis
Motivated by the potential of electrochemical techniques to analyze hybridization events fast and in a simple and cost‐effective way we present here a detection system allowing a parallel electrochemical DNA analysis. For this purpose different probe DNA strands have been immobilized on one electrode. By the use of two different target DNA sequences, both marked with the redox active methylene blue, we can show that hybridization with the complementary probe sh“NA strands can occur without steric hindrance. Each target has been recognized down to 3nM with a very high specificity of the sensor. In addition, we can detect two different ssDNA targets labeled with different redox active molecules, methylene blue and ferrocene, on one sensor surface simultaneously.