A Biosensor Composed of Glucose Oxidase‐Containing Liposomes and MnO 2 ‐Based Layered Nanocomposite | Zendy

Yoshimoto Makoto | Zendy; Iida Chihiro | Zendy; Kariya Asuka | Zendy; Takaki Noriyuki | Zendy; Nakayama Masaharu | Zendy

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A Biosensor Composed of Glucose Oxidase‐Containing Liposomes and MnO 2 ‐Based Layered Nanocomposite

Author(s) -

Yoshimoto Makoto,

Iida Chihiro,

Kariya Asuka,

Takaki Noriyuki,

Nakayama Masaharu

Publication year - 2010

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.200900413

Subject(s) - glucose oxidase , biosensor , liposome , nanocomposite , manganese oxide , manganese , chemistry , oxide , molecule , immobilized enzyme , nuclear chemistry , inorganic chemistry , materials science , chemical engineering , enzyme , nanotechnology , organic chemistry , engineering

Glucose oxidase (GOD) was encapsulated in liposomes, and then the GOD‐containing liposomes were immobilized to a MnO 2 ‐based multilayered nanocomposite film grown electrochemically. Oxidation of glucose took place on the encapsulated GOD in the manganese oxide film, and the generated H 2 O 2 molecules were oxidized catalytically at high valent Mn sites (4+) in the film. Anodic currents due to reoxidation of the reduced Mn ions (3+) were in proportion to the concentration of glucose from 19.6 to 107.1 mM. Such a simple construction of biosensor is applicable to a variety of combinations of liposomal enzymes and substrates.

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