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A Biosensor Composed of Glucose Oxidase‐Containing Liposomes and MnO 2 ‐Based Layered Nanocomposite
Author(s) -
Yoshimoto Makoto,
Iida Chihiro,
Kariya Asuka,
Takaki Noriyuki,
Nakayama Masaharu
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900413
Subject(s) - glucose oxidase , biosensor , liposome , nanocomposite , manganese oxide , manganese , chemistry , oxide , molecule , immobilized enzyme , nuclear chemistry , inorganic chemistry , materials science , chemical engineering , enzyme , nanotechnology , organic chemistry , engineering
Glucose oxidase (GOD) was encapsulated in liposomes, and then the GOD‐containing liposomes were immobilized to a MnO 2 ‐based multilayered nanocomposite film grown electrochemically. Oxidation of glucose took place on the encapsulated GOD in the manganese oxide film, and the generated H 2 O 2 molecules were oxidized catalytically at high valent Mn sites (4+) in the film. Anodic currents due to reoxidation of the reduced Mn ions (3+) were in proportion to the concentration of glucose from 19.6 to 107.1 mM. Such a simple construction of biosensor is applicable to a variety of combinations of liposomal enzymes and substrates.