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Electrochemically Induced Uptake and Release of Iron by Recombinant Horse H‐Chain Ferritin Covalently Immobilized on 6‐Mercaptohexanoic Acid Modified Gold Electrodes
Author(s) -
Scott Brandon L.,
Zapien Donald C.
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900390
Subject(s) - ferritin , covalent bond , electrochemistry , chemistry , cyclic voltammetry , ferrous , oxidizing agent , monolayer , electrode , inorganic chemistry , nuclear chemistry , biochemistry , organic chemistry
Ferritin composed entirely of the H‐chain subunit (rHF) was studied to determine whether electrochemical potential alone could induce rHF to take up or release iron. Horse rHF was expressed in E. coli , purified, and covalently immobilized on a self‐assembled monolayer of 6‐mercaptohexanoic acid on gold electrodes. Cyclic voltammetry was used to show that iron was released from rHF following electrochemical reduction, and iron uptake was induced by applying an oxidizing potential to rHF in the presence of ferrous ion.