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Ultrasensitive Eletrogenerated Chemiluminescence Immunoassay by Magnetic Nanobead Amplification
Author(s) -
Li Mingyue,
Sun Yuming,
Chen Li,
Li Lu,
Zou Guizheng,
Zhang Xiaoli,
Jin Wenrui
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900351
Subject(s) - chemiluminescence , detection limit , carcinoembryonic antigen , electrochemiluminescence , immunoassay , chemistry , chemiluminescent immunoassay , luminescent measurements , linear range , chromatography , analytical chemistry (journal) , electrode , luminescence , materials science , optoelectronics , medicine , antibody , cancer , immunology , biology
An ultrasensitive electrogenerated chemiluminescence (ECL) immunoassay was proposed by using magnetic nanobeads (MNBs) as the carrier of ECL labels for ECL emission amplification. Carcinoembryonic antigen (CEA) and MNBs were initially immobilized on a platform in 1 : 1 molar ratio via sandwich immunoreaction. Subsequently, the MNBs were released from the platform and labeled with Ru(bpy) 3 2+ species. After the MNBs with Ru(bpy) 3 2+ were immobilized on an Au electrode, ECL of the Ru(bpy) 3 2+ was measured for CEA determination. A linear relation between the ECL intensity and CEA concentration was obtained in a range of 1×10 −14 to 3×10 −13 mol/L (2.0 to 60 pg/mL) with a limit of detection of 8.0×10 −15 mol/L (1.6 pg/mL).