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Boron‐Doped Diamond Electrode Coupled to Liquid Chromatography: Application to Simultaneous Determination of Benzodiazepines
Author(s) -
Martins Isarita,
de Souza Canaes Larissa,
Doretto Keity Margareth,
Rath Susanne
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900346
Subject(s) - amperometry , repeatability , electrode , chromatography , acetonitrile , materials science , diamond , high performance liquid chromatography , analytical chemistry (journal) , chemistry , electrochemistry , composite material
Abstract The applicability of boron‐doped diamond (BDD) as a working electrode in an amperometric cell, coupled to HPLC, was demonstrated, for determining benzodiazepines in pharmaceutical preparations. The separation of the benzodiazepines was achieved using a Waters XTerra RP18 column (250×4.6 mm, 5 μm) with a mobile phase sodium phosphate (pH 3.5; 0.10 mol L −1 )‐acetonitrile (65 : 35, v/v) at flow of 1.2 mL min −1 . The measurements were performed in a system 871 Advanced Bioscan (Metrohm) with a BDD (8000 ppm) adapted to the thin layer mode cell. Stainless steel and platinum wire were used as reference and auxiliary electrodes, respectively. The cell was operated in pulse mode, using −1.9 V as initial potential. The method presented linearity, repeatability and ruggedness and it represents a novel, alternative electroanalytical method for benzodiazepines determination.