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Flow‐Injection Amperometric Method for Indirect Determination of Dopamine in the Presence of a Large Excess of Ascorbic Acid
Author(s) -
Gimenes Denise Tofanello,
dos Santos Wallans Torres Pio,
Tormin Thiago Faria,
Munoz Rodrigo Alejandro Abarza,
Richter Eduardo Mathias
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900331
Subject(s) - ascorbic acid , amperometry , detection limit , chemistry , glassy carbon , electrode , chromatography , working electrode , dopamine , analytical chemistry (journal) , electrochemistry , cyclic voltammetry , food science , neuroscience , biology
A simple flow injection amperometric system for fast and indirect quantification of dopamine (DA) in the presence of a large excess of ascorbic acid (AA) is reported. The method consists of the application of three sequential potential pulses to an unmodified glassy carbon‐working electrode positioned in a wall‐jet cell. DA is indirectly detected at +0.35 V through the reduction of the oxidation product ( o ‐dopaminoquinone) electrochemically generated at +0.80 V. The third potential pulse (0.00 V) is applied for the regeneration (cleaning) of the unmodified working electrode. The limits of detection ( LOD ) and quantification ( LOQ ) were calculated as 50 and 170 nmol L −1 , respectively. Considering the LOQ value, the present methodology allows DA quantification in the presence up to 5000‐fold more of AA (1.0 mmol L −1 ).