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A Simple Fabrication Method for Three‐Dimensional Gold Nanoparticle Electrodes and Their Application to the Study of the Direct Electrochemistry of Cytochrome c
Author(s) -
Murata Kenichi,
Kajiya Kazuki,
Nukaga Megumi,
Suga Yosuke,
Watanabe Toshiyuki,
Nakamura Nobuhumi,
Ohno Hiroyuki
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900323
Subject(s) - electrode , monolayer , colloidal gold , materials science , electrochemistry , nanoparticle , nanostructure , nanotechnology , cytochrome c , electron transfer , bioelectrochemistry , chemical engineering , chemistry , photochemistry , biochemistry , engineering , mitochondrion
A simple method for constructing gold nanoparticle‐modified electrodes with three‐dimensional nanostructures is demonstrated. The electrodes were prepared by casting citrate‐reduced AuNPs onto polycrystalline gold electrodes. The resultant electrodes had a large surface area‐to‐volume ratio, adequate for high protein loading and conferring high stability. The gold nanoparticle electrodes were covered with a self‐assembled monolayer of 11‐mercaptoundecanoic acid for electrostatic immobilization of cytochrome c (cyt c ). At the electrode, direct, reversible electron transfer from cyt c was observed with remarkable stability. Moreover, an extremely high surface coverage of electrochemically active cyt c , 167 fully packed monolayers, was obtained through use of the electrode.