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Electrocatalytic Oxidation of Glucose by the Glucose Oxidase Immobilized in Graphene‐Au‐Nafion Biocomposite
Author(s) -
Zhou Kangfu,
Zhu Yihua,
Yang Xiaoling,
Li Chunzhong
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900321
Subject(s) - biosensor , glucose oxidase , graphene , nafion , biocomposite , cyclic voltammetry , materials science , detection limit , redox , biocompatibility , electrochemistry , hydroquinone , catalysis , chemical engineering , electrode , nuclear chemistry , chemistry , inorganic chemistry , nanotechnology , chromatography , organic chemistry , composite number , composite material , engineering , metallurgy
Graphene was successfully prepared and well separated to individual sheets by introducing SO 3 − . XRD and TEM were employed to characterize the graphene. UV‐visible absorption spectra indicated that glucose oxidase (GOx) could keep bioactivity well in the graphene‐Au biocomposite. To construct a novel glucose biosensor, graphene, Au and GOx were co‐immobilized in Nafion to further modify a glassy carbon electrode (GCE). Electrochemical measurements were carried out to investigate the catalytic performance of the proposed biosensor. Cyclic voltammograms (CV) showed the biosensor had a typical catalytic oxidation response to glucose. At the applied potential +0.4 V, the biosensor responded rapidly upon the addition of glucose and reached the steady state current in 5 s, with the present of hydroquinone. The linear range is from 15 μM to 5.8 mM, with a detection limit 5 μM (based on the S / N =3). The Michaelis‐Menten constant was calculated to be 4.4 mM according to Lineweaver–Burk equation. In addition, the biosensor exhibits good reproducibility and long‐term stability. Such impressive properties could be ascribed to the synergistic effect of graphene‐Au integration and good biocompatibility of the hybrid material.