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Fabrication of a Sensitive Cholesterol Biosensor Based on Cobalt‐oxide Nanostructures Electrodeposited onto Glassy Carbon Electrode
Author(s) -
Salimi Abdollah,
Hallaj Rahman,
Soltanian Saied
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900229
Subject(s) - cholesterol oxidase , biosensor , detection limit , cyclic voltammetry , nafion , calibration curve , materials science , cobalt , electrode , cobalt oxide , glassy carbon , oxide , flow injection analysis , electrochemistry , analytical chemistry (journal) , nuclear chemistry , chemistry , inorganic chemistry , nanotechnology , chromatography , cholesterol , biochemistry , metallurgy
Electrodeposited cobalt oxide (CoOx) nanomaterials are not only used for immobilization of cholesterol oxidase (ChOx) but also as electron transfer mediator for oxidation of H 2 O 2 generated in the enzymatic reaction. Voltammetry and flow injection analysis (FIA) were used for determination of cholesterol. FIA determination of cholesterol with biosensors yielded a calibration curve with the following characteristics: linear range up to 50 μM, sensitivity of 43.5 nA μM −1 cm −2 and detection limit of 4.2 μM. The apparent Michaelis‐Menten constant and the response time of the biosensor are 0.49 mM and 15 s, respectively. This biosensor also exhibits good stability, reproducibility and long life time.