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The Comparison of Electrochemical Assay and Agarose Gel Electrophoresis for the Determination of DNA Damage Induced by Kainic Acid
Author(s) -
Karadeniz Hakan,
Armagan Guliz,
Erdem Arzum,
Turunc Ezgi,
Caliskan Ayfer,
Kanit Lutfiye,
Yalcin Ayfer
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900218
Subject(s) - agarose gel electrophoresis , guanine , kainic acid , agarose , dna , gel electrophoresis , dna damage , microbiology and biotechnology , chemistry , in vivo , electrophoresis , genomic dna , in vitro , chromatography , biochemistry , biology , gene , genetics , nucleotide , receptor , glutamate receptor
A possible DNA damage after interaction of kainic acid (KA) with calf thymus double stranded DNA and genomic DNA was herein determined in in vitro and in vivo conditions using; electrochemical assay and agarose gel electrophoresis. The changes in guanine signal were detected as an indicator of DNA damage in genomic DNA samples isolated from 1 or 10 mg/kg KA‐treated animals. The decreased levels of guanine signal were found as 29% and 33% by 1 and 10 mg/kg KA treatment when compared to controls, respectively. The results of gel electrophoresis confirmed DNA damage obtained in identical samples by electrochemical method.