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Simultaneous Determination of Tyrosine and Histidine by Differential Pulse Cathodic Stripping Voltammetry Using H‐point Standard Addition Method in Tap and Seawater
Author(s) -
Shahlaei Mohsen,
Gholivand Mohammad Bagher,
Pourhossein Alireza
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900200
Subject(s) - hanging mercury drop electrode , chemistry , histidine , cathodic stripping voltammetry , standard addition , tyrosine , copper , voltammetry , analytical chemistry (journal) , detection limit , seawater , tap water , chromatography , inorganic chemistry , electrode , electrochemistry , amino acid , organic chemistry , biochemistry , engineering , environmental engineering , oceanography , geology
H‐point standard addition method (HPSAM) has been applied for simultaneous determination of tyrosine and histidine in trace levels using copper ions by adsorptive cathodic stripping voltammetry. The amino acids‐Cu(II) complexes were accumulated onto the surface of a hanging mercury drop electrode for 40 s. The reduction peaks of preconcentrated complexes were used for simultaneous determination of amino acids in the range 8.0–180 and 30–1100 nM for tyrosine and histidine respectively. The effect of various parameters such as pH, concentration of copper, accumulation time and scan rate on the selectivity were studied. Under the optimized conditions the method was successfully applied for determination of tyrosine and histidine in synthetic and real samples.