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Lactate Biosensor Based on Hydrotalcite‐Like Compounds: Performances and Application to Serum Samples
Author(s) -
Carpani Irene,
Guadagnini Lorella,
Tonelli Domenica
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200900118
Subject(s) - biosensor , hydrotalcite , detection limit , hydrogen peroxide , chemistry , platinum , electrode , chromatography , nuclear chemistry , catalysis , organic chemistry , biochemistry
Abstract A lactate biosensor based on lactate oxidase supported onto a hydrotalcite, electrochemically deposited on a platinum surface, was developed for the first time. For the best electrode configuration, a linear response up to 0.8 mM, with a limit of detection of 14 μM and a sensitivity of 91 mA M −1 cm −2 , was obtained. The influence of some interferents due to the oxidation of hydrogen peroxide (at +0.35 V vs. SCE) was also studied. By controlling carefully the experimental conditions, the determination of lactate in a commercial serum sample in the presence of interferents was successfully accomplished.