Amperometric Ethanol Biosensor Based on Integration of Alcohol Dehydrogenase with Meldola's Blue/Ordered Mesoporous Carbon Electrode | Zendy

Jiang Xiaoyan | Zendy; Zhu Liande | Zendy; Yang Dongxu | Zendy; Mao Xuyan | Zendy; Wu Yanhui | Zendy

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Amperometric Ethanol Biosensor Based on Integration of Alcohol Dehydrogenase with Meldola's Blue/Ordered Mesoporous Carbon Electrode

Author(s) -

Jiang Xiaoyan,

Zhu Liande,

Yang Dongxu,

Mao Xuyan,

Wu Yanhui

Publication year - 2009

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.200804586

Subject(s) - detection limit , biosensor , amperometry , nicotinamide adenine dinucleotide , alcohol dehydrogenase , chemistry , nuclear chemistry , electrode , linear range , electrocatalyst , carbon fibers , ethanol , inorganic chemistry , chromatography , materials science , nad+ kinase , organic chemistry , electrochemistry , composite number , biochemistry , enzyme , composite material

An amperometric ethanol biosensor was fabricated by integration of alcohol dehydrogenase (ADH) with meldola's blue (MB)/ordered mesoporous carbon (OMC) composite modified glassy carbon electrode (MB/OMC/GCE). The MB/OMC/GCE was highly sensitive for nicotinamide adenine dinucleotide (NADH) measurement (9.1±0.25 μA/mM) and gave a low detection limit of 0.21±0.02 μM. The ethanol biosensor exhibited a wide linear range up to 6 mM with a lower detection limit of 19.1±0.58 μM as well as a high sensitivity of 34.58±2.43 nA/mM without suffering any interference from some common electroactive compounds.

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