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Amperometric Ethanol Biosensor Based on Integration of Alcohol Dehydrogenase with Meldola's Blue/Ordered Mesoporous Carbon Electrode
Author(s) -
Jiang Xiaoyan,
Zhu Liande,
Yang Dongxu,
Mao Xuyan,
Wu Yanhui
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804586
Subject(s) - detection limit , biosensor , amperometry , nicotinamide adenine dinucleotide , alcohol dehydrogenase , chemistry , nuclear chemistry , electrode , linear range , electrocatalyst , carbon fibers , ethanol , inorganic chemistry , chromatography , materials science , nad+ kinase , organic chemistry , electrochemistry , composite number , biochemistry , enzyme , composite material
An amperometric ethanol biosensor was fabricated by integration of alcohol dehydrogenase (ADH) with meldola's blue (MB)/ordered mesoporous carbon (OMC) composite modified glassy carbon electrode (MB/OMC/GCE). The MB/OMC/GCE was highly sensitive for nicotinamide adenine dinucleotide (NADH) measurement (9.1±0.25 μA/mM) and gave a low detection limit of 0.21±0.02 μM. The ethanol biosensor exhibited a wide linear range up to 6 mM with a lower detection limit of 19.1±0.58 μM as well as a high sensitivity of 34.58±2.43 nA/mM without suffering any interference from some common electroactive compounds.