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Differential Pulse Voltammetric Detection of Hepatitis C Virus 1a Oligonucleotide Chain by a Label‐Free Electrochemical DNA Hybridization Biosensor Using Consensus Sequence of Hepatitis C Virus 1a Probe on the Pencil Graphite Electrode
Author(s) -
PournaghiAzar M. H.,
Ahour F.,
Hejazi M. S.
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804570
Subject(s) - differential pulse voltammetry , biosensor , oligonucleotide , guanine , detection limit , dna , oligomer restriction , chemistry , hybridization probe , dna–dna hybridization , microbiology and biotechnology , cytosine , electrode , electrochemistry , analytical chemistry (journal) , cyclic voltammetry , biochemistry , biology , nucleotide , chromatography , gene
The short sequence related to hepatitis C virus (HCV1) is detected by a label‐free DNA hybridization biosensor. The sensor relies on the immobilization of a 20‐mer oligonucleotide containing 2 guanine and 11 cytosine bases denoted PHCV1 as probe on the pencil graphite electrode (PGE). The hybridization event was monitored by differential pulse voltammetry (DPV) using the guanine signal. The selectivity of the biosensor was studied using some noncomplementary oligonucleotides. Diagnostic performance of the biosensor is described and the detection limit was found to be 6.5 nM.