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A Simple and Sensitive Biosensor Based on Silver Enhancement of Aptamer‐Gold Nanoparticle Aggregation
Author(s) -
Zhang Zhen,
Chen Chunlai,
Sheng Zhao Xin
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804562
Subject(s) - aptamer , colloidal gold , biosensor , nanotechnology , silver nanoparticle , nanoparticle , naked eye , combinatorial chemistry , materials science , chemistry , chromatography , detection limit , microbiology and biotechnology , biology
Abstract We have found that dispersed gold nanoparticles (AuNPs) have higher silver enhancement than that of aggregated AuNPs. Based on this observation we propose a general methodology of assay, the silver enhancement of aptamer‐gold nanoparticle aggregation (SEAGNA). The assay is simple, inexpensive, fast, and sensitive. Adenosine aptamer is used to demonstrate the essence of this method. A tiny amount of sample is enough, and 0.1 pmol (0.1 μM in 1 μL) of adenosine can be detected with naked eyes. The sensitivity of most existing AuNPs‐based colorimetric biosensors can be easily improved by applying our silver enhancement.