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Molecularly Imprinted Polymerized Methylene Green as a Platform for Electrochemical Sensing of Aptamer–Thrombin Interactions
Author(s) -
Evtugyn Gennady,
Porfireva Anna,
Ivanov Alexey,
Konovalova Olga,
Hianik Tibor
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804556
Subject(s) - aptamer , dielectric spectroscopy , detection limit , methylene blue , adsorption , polymerization , biosensor , electrochemistry , chemistry , analytical chemistry (journal) , materials science , chemical engineering , electrode , nanotechnology , chromatography , organic chemistry , polymer , catalysis , genetics , photocatalysis , engineering , biology
The biosensors based on DNA aptamers immobilized by electrostatic adsorption onto electropolymerized Methylene Green (MG)) imprinted with DNA have been developed and examined for thrombin detection in the range from 1 nM to 1 μM using electrochemical impedance spectroscopy (EIS) and potentiometry. The participation of DNA in MG electropolymerization and imprints formation was proved by measurements of EIS and redox signals of neutral and charged indicators. Changes in the morphology of the layers were examined using atomic force microscopy (AFM). The addition of DNA at the electropolymerization stage followed by acidic treatment of the coating significantly improved the efficiency of electrostatic adsorption of the DNA aptamer and provided sensitive detection of thrombin with limit of detection 0.5 nM.