Chloroperoxidase Modified Electrode for Amperometric Determination of 2,4,6‐Trichlorophenol | Zendy

DíazDíaz Goretti | Zendy; BlancoLópez M. Carmen | Zendy; LoboCastañón M. Jesús | Zendy; MirandaOrdieres Arturo J. | Zendy; TuñónBlanco Paulino | Zendy

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Chloroperoxidase Modified Electrode for Amperometric Determination of 2,4,6‐Trichlorophenol

Author(s) -

DíazDíaz Goretti,

BlancoLópez M. Carmen,

LoboCastañón M. Jesús,

MirandaOrdieres Arturo J.,

TuñónBlanco Paulino

Publication year - 2009

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.200804545

Subject(s) - amperometry , chemistry , detection limit , biosensor , cyclic voltammetry , electrode , chromatography , graphite , benzoquinone , selectivity , analyte , inorganic chemistry , electrochemistry , organic chemistry , catalysis , biochemistry

A carbon paste‐poly( o ‐phenylendiamine)‐modified electrode to be used as amperometric biosensor for 2,4,6‐trichlorophenol (TCP) is described. The enzyme chloroperoxidase (EC 1.11.1.10) from Caldariomyces fumago is immobilized through dispersion in a graphite paraffin oil carbon paste covered by an electrogenerated poly( o ‐phenylendiamine) (PPD) layer. The main enzymatic dehalogenation product, 2,6‐dichloro‐1,4‐benzoquinone (DCQ) is characterized by liquid chromatography‐mass spectrometry and cyclic voltammetry. This product is electrochemically active and can be detected amperometrically at +150 mV vs. Ag|AgCl|KCl (3 M). The biosensor exhibits a response time of 4 min, a detection limit of 10 −7 M, and a dynamic linear range between 10 −7 and 10 −6 M. Selectivity as well as operating and storage stability were evaluated.

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