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Chloroperoxidase Modified Electrode for Amperometric Determination of 2,4,6‐Trichlorophenol
Author(s) -
DíazDíaz Goretti,
BlancoLópez M. Carmen,
LoboCastañón M. Jesús,
MirandaOrdieres Arturo J.,
TuñónBlanco Paulino
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804545
Subject(s) - amperometry , chemistry , detection limit , biosensor , cyclic voltammetry , electrode , chromatography , graphite , benzoquinone , selectivity , analyte , inorganic chemistry , electrochemistry , organic chemistry , catalysis , biochemistry
A carbon paste‐poly( o ‐phenylendiamine)‐modified electrode to be used as amperometric biosensor for 2,4,6‐trichlorophenol (TCP) is described. The enzyme chloroperoxidase (EC 1.11.1.10) from Caldariomyces fumago is immobilized through dispersion in a graphite paraffin oil carbon paste covered by an electrogenerated poly( o ‐phenylendiamine) (PPD) layer. The main enzymatic dehalogenation product, 2,6‐dichloro‐1,4‐benzoquinone (DCQ) is characterized by liquid chromatography‐mass spectrometry and cyclic voltammetry. This product is electrochemically active and can be detected amperometrically at +150 mV vs. Ag|AgCl|KCl (3 M). The biosensor exhibits a response time of 4 min, a detection limit of 10 −7 M, and a dynamic linear range between 10 −7 and 10 −6 M. Selectivity as well as operating and storage stability were evaluated.