Premium
Electrochemical Detection of DNA Melting Curves by Means of Heated Biosensors
Author(s) -
Surkus AnnetteEnrica,
Flechsig GerdUwe
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804539
Subject(s) - osmium tetroxide , biosensor , electrode , oligonucleotide , nucleic acid , melting curve analysis , nucleic acid thermodynamics , analytical chemistry (journal) , electrochemistry , peptide nucleic acid , materials science , chemistry , dna , nanotechnology , chromatography , base sequence , biochemistry , polymerase chain reaction , physics , electron microscope , optics , gene
This article reports about the detection of DNA melting curves at heated electrochemical biosensors. Osmium tetroxide‐bipyridine‐labeled target oligonucleotides are hybridized with probe oligonucleotides immobilized on gold electrodes. Then, the gold electrode is successively heated in order to measure a complete melting curve consisting of alternating current voltammetric signals. Melting temperatures ϑ m , determined at various ionic strengths and in dependence on different numbers of base pair mismatches, have been compared with those obtained by means of UV spectrophotometry. The proposed method holds great promise for the fast and easy parallel detection of nucleic acids sequences on selectively heated electrode arrays. A stringent hybridization temperature can be easily adjusted in order to discriminate base pair mismatches.