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Synthesis and Characterization of Poly(toluidine blue) Nanowires and Their Application in Amperometric Biosensors
Author(s) -
Lu LiMin,
Wang SuiPing,
Qu FengLi,
Zhang XiaoBing,
Huan Shuangyan,
Shen GuoLi,
Yu RuQin
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804532
Subject(s) - amperometry , horseradish peroxidase , toluidine , scanning electron microscope , electrode , biosensor , materials science , detection limit , transmission electron microscopy , electrochemistry , nanowire , electron transfer , analytical chemistry (journal) , nuclear chemistry , chemical engineering , nanotechnology , chemistry , chromatography , organic chemistry , composite material , engineering , enzyme
Abstract Poly(toluidine blue) nanowires (PTBNWs) with an average diameter of ca. 200 nm and length of ca. 5 μm were synthesized for the first time using a template‐directed electropolymerization strategy with a nanopore polycarbonate (PC) membrane template. Their morphological characterization was carried out by scanning electron microscopy (SEM) and transmission electron microscope (TEM). By electrochemical polymerization, horseradish peroxidase (HRP) was encapsulated in situ in PTBNWs (denoted as PTBNWs‐HRP) for potential biosensor applications. PTBNWs‐HRP was then modified on a glassy carbon (GC) electrode. In the system obtained, the PTBNWs served as an excellent redox mediator and exhibited high efficiency of electron transfer between the HRP and the GC electrode for the reduction of H 2 O 2 . The proposed electrode can be used as an excellent amperometric sensor for H 2 O 2 at −0.1 V with a linear response range covering from 1 μM to 28 mM, a detection limit of 1 μM (based on S/N=3) and a fast response time of less than 8 s.