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Reagentless Biosensor for Hydrogen Peroxide Based on the Immobilization of Hemoglobin in Platinum Nanoparticles Enhanced Poly(chloromethyl thiirane) Cross‐linked Chitosan Hybrid Film
Author(s) -
Jia Shanshan,
Fei Junjie,
Tian Tian,
Zhou Fangqin
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804531
Subject(s) - hydrogen peroxide , biosensor , chitosan , chemistry , nanoparticle , electrochemistry , platinum nanoparticles , platinum , redox , nuclear chemistry , combinatorial chemistry , inorganic chemistry , polymer chemistry , materials science , electrode , nanotechnology , organic chemistry , catalysis , biochemistry
An unmediated hydrogen peroxide (H 2 O 2 ) biosensor was prepared by co‐immobilizing hemoglobin (Hb) with platinum nanoparticles enhanced poly(chloromethyl thiirane) cross‐linked chitosan (CCCS‐PNs) hybrid film. CCCS could provide a biocompatible microenvironment for Hb and PNs could accelerate the electron transfer between Hb and the electrode. Spectroscopic analysis indicated that the immobilized Hb could maintain its native structure in the CCCS‐PNs hybrid film. Entrapped Hb exhibited direct electrochemistry for its heme Fe(III)/Fe(II) redox couples at −0.396 V in the CCCS‐PNs hybrid film, as well as peroxidase‐like activity to the reduction of hydrogen peroxide without the aid of an electron mediator.