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Nerve Agents Assay Using Cholinesterase Based Biosensor
Author(s) -
Pohanka Miroslav,
Dobes Petr,
Drtinova Lucie,
Kuča Kamil
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804528
Subject(s) - sarin , nerve agent , tabun , soman , acetylcholinesterase , acetylthiocholine , chemistry , biosensor , cholinesterase , chromatography , detection limit , aché , enzyme , biochemistry , pharmacology , medicine
Electrochemical biosensor based on electric eel acetylcholinesterase (AChE) (EC 3.1.1.7) was performed for assay of nerve agents – tabun, sarin, soman, cyclosarin, and VX. The biosensor used AChE as biorecognition element. The presence of nerve agents was accompanied by a strong inhibition of AChE activity. Enzyme activity is easily measurable by electrochemical oxidation of thiocholine created from acetylthiocholine (ATChCl) by AChE‐catalyzed hydrolysis. The tested nerve agents were successfully assayed. The best limits of detection were achieved for sarin (5.88×10 −10 M) and VX (8.51×10 −10 M) after one‐minute assay. The biosensor was found long term stable at low as well as laboratory temperature.