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Disposable Electrodes for Capacitive Immunosensor
Author(s) -
Teeparuksapun Kosin,
Kanatharana Proespichaya,
Limbut Warakorn,
Thammakhet Chongdee,
Asawatreratanakul Punnee,
Mattiasson Bo,
Wongkittisuksa Booncharoen,
Limsakul Chusak,
Thavarungkul Panote
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804517
Subject(s) - human serum albumin , detection limit , electrode , linear range , materials science , chromatography , biosensor , analytical chemistry (journal) , chemistry , nanotechnology
Disposable electrodes were fabricated by coating chromium (5 nm) and gold (200 nm) on glass strips (5.0 mm×25.4 mm) and used in a label‐free immunosensor. Human serum albumin (HSA) and its antigen (anti‐HSA) were used as a model system. Electropolymerization of o ‐phenylenediamine was used for the immobilization of anti‐HSA by covalent binding. A linear relationship was obtained in the range from 1.0×10 −14 to 1.0×10 −9 M with a limit of detection of 8.0×10 −15 M. Each modified electrode can be reused up to 30 times. The developed system was applied for human serum samples and compared to Albumin BCG method.