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A Sandwich‐Type Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene Using Locked Nucleic Acids on Gold Electrode
Author(s) -
Wang Kun,
Chen Jinghua,
Chen Jing,
Liu Ailin,
Li Guangwen,
Luo Hongbin,
Lin Xinhua,
Chen Yuanzhong
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804507
Subject(s) - biosensor , avidin , nucleic acid , dna , electrode , chemistry , hybridization probe , biotinylation , chronic myelogenous leukemia , biotin , microbiology and biotechnology , chromatography , combinatorial chemistry , biochemistry , biology , leukemia , genetics
In this study, a sandwich‐type electrochemical enzyme‐based LNA‐modified DNA biosensor was developed to detect relative gene in chronic Myelogenous Leukemia first. This biosensor is based on a ‘sandwich’ detection strategy, which involves a pair of probes (a capture probe immobilized at the electrode surface and a reporter probe labeled biotin as an affinity tag for avidin‐HRP) modified LNA. Since biotin can be connected with avidin‐HRP, this biosensor offers an enzymatically amplified electrochemical current signal for the detection of target DNA. This new pattern exhibits high sensitivity and selectivity, and this biosensor has been used for an assay of PCR real sample with satisfactory result.