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Amperometric Biosensor Based on Immobilization of Oxalate Oxidase in a Mucin/Chitosan Matrix
Author(s) -
Benavidez Tomás E.,
Capra Raúl H.,
Alvarez Cecilia I.,
Baruzzi Ana M.
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804482
Subject(s) - glutaraldehyde , chitosan , biosensor , chemistry , oxalate , amperometry , immobilized enzyme , chromatography , glucose oxidase , nuclear chemistry , matrix (chemical analysis) , electrode , enzyme , biochemistry , inorganic chemistry , electrochemistry
An amperometric electrode for oxalate determination immobilizing the oxalate oxidase in a mucin/chitosan (muc/chit) gel with glutaraldehyde as crosslinking agent is presented. The effect of muc/chit weight ratio and volume percent (vol.%) of glutaraldehyde was studied. A very low dynamic response was observed in the case of 100% chitosan with 5 vol.% crosslinking agent. The addition of mucin to chitosan for enzyme immobilization resulted in a biosensor with much better performance, concerning to dynamic response, sensitivity, and stability, with 75% of the initial response after two months. The ratio muc/chit 70/30 was considered optimum for the immobilization. A slight crosslinking and the incorporation of mucin largely influences the swelling and diffusion of the analyte; a direct effect of these properties on the calibration slope was found; the hydrophilic environment for the biomolecule also favor the enzymatic activity through a higher enzyme‐substrate interaction.