Premium
A Novel Hydrogen Peroxide Biosensor Based on the Synergistic Effect of Gold‐Platinum Alloy Nanoparticles/Polyaniline Nanotube/Chitosan Nanocomposite Membrane
Author(s) -
Wang Xueliang,
Yang Tao,
Feng Yuanyuan,
Jiao Kui,
Li Guicun
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804479
Subject(s) - biosensor , nanocomposite , dielectric spectroscopy , detection limit , cyclic voltammetry , polyaniline , platinum nanoparticles , hydrogen peroxide , materials science , amperometry , biocompatibility , nuclear chemistry , nanotube , carbon nanotube , nanoparticle , chitosan , colloidal gold , chemical engineering , platinum , chemistry , electrochemistry , electrode , nanotechnology , catalysis , chromatography , organic chemistry , polymer , composite material , engineering , metallurgy , polymerization
Based on the immobilization of horseradish peroxidase (HRP) in chitosan(CS) on a glassy carbon electrode (GCE) modified with the Au‐Pt alloy nanoparticles (NPs) / polyaniline nanotube (nanoPAN) nanocomposite film, a novel hydrogen peroxide biosensor was constructed. The modified processes of GCE were monitored by cyclic voltammetry and electrochemical impedance spectroscopy. Au‐PtNPs/nanoPAN/CS had a better synergistic electrochemical effect than did AuNPs/nanoPAN/CS or PtNPs/nanoPAN/CS. The amperometric response of the biosensor towards H 2 O 2 was investigated by successively adding aliquots of H 2 O 2 to a continuous stirring phosphate buffer solution under the optimized conditions. Because Au‐PtNPs have unique catalytic properties and good biocompatibility, and especially Au‐PtNPs and nanoPAN have synergistic augmentation for facilitating electron‐transfer, the biosensor displayed a fast response time (<2 s) and broad linear response to H 2 O 2 in the range from 1.0 to 2200 μmol L −1 with a relatively low detection limit of 0.5 μmol L −1 at 3 times the background noise. Moreover, the biosensor can be applied in practical analysis and exhibited high sensitivity, good reproducibility, and long‐term stability.