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A Novel NH 2 /ITO Ion Implantation Electrode: Preparation, Characterization, and Application in Bioelectrochemistry
Author(s) -
Cao Wei,
Hu Jingbo,
Li Qilong,
Fang Weihai
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804470
Subject(s) - cyclic voltammetry , electrode , dielectric spectroscopy , indium tin oxide , bioelectrochemistry , chemistry , electrochemistry , inorganic chemistry , tin oxide , analytical chemistry (journal) , adsorption , hydrogen peroxide , nuclear chemistry , oxide , chromatography , organic chemistry
A novel NH 2 + ion implantation‐modified indium tin oxide (NH 2 /ITO) electrode was prepared. Acid‐pretreated, negatively charged MWNTs were firstly modified on the surface of NH 2 + ion implantation electrode, then, positively charged Mb was adsorbed onto MWNTs films by electrostatic interaction. The assembly of MWNTs and Mb was characterized with electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The immobilized Mb showed a couple of quasireversible cyclic voltammetry peaks in pH 7.0 phosphate buffer solution (PBS). The apparent surface concentration of Mb at the electrode surface was 1.06×10 −9 mol cm −2 . The Mb/MWNTs/NH 2 /ITO electrode also gave an improved electrocatalytic activity towards the reduction of hydrogen peroxide. The catalysis currents increased linearly to the H 2 O 2 concentration in a wide range from 9×10 −7 to 9.2×10 −5 M with a correlation coefficient of 0.999. The detection limit was 9.0×10 −7 M. The experiment results demonstrated that the modified electrode provided a biocompatible microenvironment for protein and supplied a necessary pathway for its direct electron transfer.