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Interactions of Dissolved dsDNA with Intercalating Drug by Anodic Voltammetry and Spectroscopy. Influence of pH
Author(s) -
Nowicka Anna M.,
Zabost Ewelina,
Klim Barbara,
Mazerska Zofia,
Stojek Zbigniew
Publication year - 2009
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200804464
Subject(s) - intercalation (chemistry) , chemistry , differential pulse voltammetry , binding constant , cyclic voltammetry , spectroscopy , voltammetry , analytical chemistry (journal) , crystallography , inorganic chemistry , binding site , nuclear chemistry , electrochemistry , electrode , chromatography , biochemistry , physics , quantum mechanics
The interactions of C‐1305 (5‐dimethylaminopropylamino‐8‐hydroxy‐6 H ‐v‐triazolo[4,5,1‐ de ]acridin‐6‐one) with DNA were studied using differential pulse voltammetry and UV‐vis spectroscopy. C‐1305 interacts with dsDNA in two ways: by intercalation and by binding to the minor‐groove. For the intercalation at physiological pH (7.4) the values of the binding constant, K 1 , and the binding‐site size, n 1 , equal 3.36×10 5 M −1 and 2.5, respectively. For the weak interactions the K 2 and n 2 parameters equal 0.18×10 5 M −1 and 4. In the presence of excess NaCl the weak interactions do not vanish, therefore they are assigned to the minor groove binding. Substantial and complex is the influence of pH.